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Mis13 Phosphorylation promotes meiotic assembly of kinetochores

• 1、Science Center for Future Foods, Jiangnan University, Wuxi, Jiangsu 214122, China
• 2、School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China

Abstract：Kinetochore disassembles during the prophase of meiosis and reassembles before meiosis I. The regulatory mechanism of this process and its impact on meiosis remain unclear. We find that Aurora B kinase mediates the phosphorylation of Mis13, thereby regulating the assembly of kinetochore during meiosis in fission yeast. During prophase of meiosis, Mis13-GFP foci largely disappear along with kinetochore disasseMis13 Phosphorylation promotes meiotic assembly of kinetochoresmbly; however, in the Mis13 phosphomimetic mutant, Mis13-GFP foci can be observed, and the centromeric localization of kinetochore proteins Mis12 and Nnf1 is significantly enhanced. When cells are arrested at the prophase of meiosis II, in the Mis13 phosphomimetic mutant, the intensity of Mis13-GFP foci is significantly enhanced; while in the Mis13 non-phosphorylatable mutant, the intensity of Mis13-GFP is significantly reduced. These results indicate that Mis13 phosphorylation promotes the kinetochore assembly during meiosis.

Keywords： Meiosis Kinetochore Microscopy Kinetochore disassembly and assembly Schizosaccharomyces pombe